Dissociation Reagents

Gibco™ Trypan Blue Solution, 0.4%

Gibco™ Trypan Blue Solution, 0.4%

Routinely used as a cell stain to assess cell viability using the dye exclusion test

Corning™ Cell Recovery Solution

Corning™ Cell Recovery Solution

Allows for the recovery of cells cultured on Corning™ Matrigel™ Basement Membrane Matrix for subsequent biochemical analyses

Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X)

Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

Gibco™ MEM Non-Essential Amino Acids Solution (100X)

Gibco™ MEM Non-Essential Amino Acids Solution (100X)

Gibco™ MEM Non-Essential Amino Acids are used as a supplement for cell culture medium, to increase cell growth and viability.

Gibco™ ACK Lysing Buffer

Gibco™ ACK Lysing Buffer

Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow

Corning™ Matrigel™ hESC-Qualified Matrix

Corning™ Matrigel™ hESC-Qualified Matrix

Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.

Gibco™ L-Glutamine (200 mM)

Gibco™ L-Glutamine (200 mM)

Ready-to-use stock solution that acts as a cell culture supplement

Corning™ Matrigel™ GFR Basement Membrane Matrix

Corning™ Matrigel™ GFR Basement Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Corning™ Cell-Tak Cell and Tissue Adhesive

Corning™ Cell-Tak Cell and Tissue Adhesive

Attach cells or tissue sections to many types of surfaces, including plastic, glass, metal, FEP Polymer, and biological materials. Corning™ Cell-Tak Cell and Tissue Adhesive is a specially formulated protein solution extracted from marine mussel, Mytilus edulis.

Gibco™ 2-Mercaptoethanol

Gibco™ 2-Mercaptoethanol

A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals

Corning™ Matrigel™ Matrix

Corning™ Matrigel™ Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Gibco™ Poloxamer 188 Non-ionic Surfactant (100X)

Gibco™ Poloxamer 188 Non-ionic Surfactant (100X)

Gibco™ Poloxamer 188 is a non-ionic surfactant used to control shear forces in suspension cultures.

Gibco™ HT Supplement (100X)

Gibco™ HT Supplement (100X)

HT-supplemented medium is suitable for post-selection rescue to overcome the effects of residual intracellular aminopterin.

Gibco™ Distilled Water

Gibco™ Distilled Water

High-quality water for use as a solvent in the preparation of cell culture media and laboratory reagents

Gibco™ Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X)

Gibco™ Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

Gibco™ HAT Supplement (50X)

Gibco™ HAT Supplement (50X)

HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT-myeloma cells

Gibco™ MEM Amino Acids Solution (50X)

Gibco™ MEM Amino Acids Solution (50X)

Used as a growth supplement for cell culture medium, to increase cell growth and viability

Gibco™ Phytohemagglutinin, M form (PHA-M)

Gibco™ Phytohemagglutinin, M form (PHA-M)

This extract is capable of inducing blastogenesis in vitro in various mammalian mononuclear cells when used at a concentration of 1 to 2% v/v

Corning™ BioCoat™ Poly-D-Lysine

Corning™ BioCoat™ Poly-D-Lysine

Recommended for the culture of neurons, glial cells, and transfected cells

Gibco™ KaryoMAX™ Potassium Chloride Solution

Gibco™ KaryoMAX™ Potassium Chloride Solution

0.075M hypotonic solution for enlarging cells to facilitate the spreading of metaphase chromosomes for karyotyping

Invitrogen™ Zymosan A (<i>S. cerevisiae</i>) BioParticles™, Alexa Fluor™ 488 conjugate

Invitrogen™ Zymosan A (S. cerevisiae) BioParticles™, Alexa Fluor™ 488 conjugate

Study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry

Corning™ Fibronectin, Human

Corning™ Fibronectin, Human

Promotes cellular attachment, spreading, and proliferation on tissue-culture surfaces. Corning™ Fibronectin, Human can be used as an additive to serum-free culture media.

Gibco™ KaryoMAX™ Giemsa Stain Solution

Gibco™ KaryoMAX™ Giemsa Stain Solution

For G-banding of chromosomes for cytogenetic analysis

Gibco™ Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X)

Gibco™ Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

Gibco™ Gurr Buffer Tablets

Gibco™ Gurr Buffer Tablets

A convenient way to prepare a pH 6.8 phosphate buffer for dilution of Giemsa stain

Gibco™ HEPES (Ultra Pure)

Gibco™ HEPES (Ultra Pure)

A zwitterionic organic chemical buffering agent commonly used in cell culture media

Gibco™ Sodium Bicarbonate 7.5% solution

Gibco™ Sodium Bicarbonate 7.5% solution

Commonly used for maintaining the pH of cell culture medium in the presence of 4 to 10% carbon dioxide

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