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40% off the Macherey Nagel NucleoSpin Gel and PCR Clean-up kit when you Buy a Taq polymerase PDF Print E-mail
Wednesday, 18 January 2012 11:24

40% off the Macherey Nagel NucleoSpin Gel and PCR Clean-up kit
 MACHEREY-NAGEL Logo



Buy Now - Call 01509555500

PCR clean-up and gel extraction
The superior 2 in 1 clean-up kit …
NucleoSpin® Gel and PCR Clean-up

Offer valid until 30th April 2012

40% off the Macherey Nagel NucleoSpin Gel and PCR Clean-up kit when you buy a Taq polymerase

Product at a glance
Sample material < 400 μL PCR reaction mix / < 400 mg TAE/TBE agarose gel
Fragment size 50 bp–20 kbp
Typical recovery 70–95 %
A260 / A280 1.8–1.9
Elution volume 15–30 μL
Preparation time 10 min / 6 preps
Binding capacity 25 μg

PCR Fragment High recovery for loew elution volumes
a PCR fragment with a size of 782bp was purified from a 1% TAE agarose gel according to the standard protocol of NucleoSpin© Extract II using different elution volumes as shown. all eluates were adjusted to 25µL loading dye. for analysis the mixture was loaded onto a 1% TAE agarose gel. Recovery was estimated against reference samples (lane 1 to 4). Even with an elution volume down to 1.5µL recovery of up to 75 to 100% can be achieved.

Procedure


Application Data
Data
Data
PCR clean-up: High recovery down to 50 bp
DNA fragments of different sizes were purified from standard PCR buffer using NucleoSpin® Gel and PCR Clean-up then eluted in different volumes (15 μL, 30 μL, 50 μL). DNA was analyzed and quantified by Agilent Bioanalyzer 2100.		 Gel extraction: High recovery in a wide range of fragment sizes
DNA fragments of different sizes were purified from 1 % TAE agarose gel by NucleoSpin® Gel and PCR Clean-up. DNA was analyzed and quantified by Agilent Bioanalyzer 2100. Fragments of 10 kbp to 20 kbp were recovered with a rate of 30–50 % (data not shown).


For more information please contact Customer Services on 01509555500 or email This e-mail address is being protected from spambots. You need JavaScript enabled to view it and order the following offer codes. The offers below are only available if a Taq polymerase is purchased on the same order; only one offer code allowed per Taq polymerase purchase. For eligible Taq polymerases see the tables below. Offer valid until 30th April 2012


Offer Code Standard Product Code Description Pack Size Offer Price
MNPROMO67 NZ740609250 NucleoSpin Gel and PCR Clean-up kit 250 preps £208.24
MNPROMO68 NZ74060950 NucleoSpin Gel and PCR Clean-up kit 50 preps £48.15


Product Code

 Description Pack Size
Routine PCR
FB6000-10 Taq DNA Polymerase (recombinant) with 10x buffer containing 15mM MgCl2 100 reactions
FB6000-45 Taq DNA Polymerase (recombinant) with 10x buffer (25mM MgCl2 provided separately) 100 reactions
10342-053 Taq DNA Polymerase, Recombinant (supplied with a 10X Taq Reaction buffer (200 mM Tris pH 8.4, 500 mM KCl) and a tube of 50 mM
MgCl2)		 100 reactions
EP0401 Taq DNA polymerase (recombinant) (supplied with 10x Taq Buffer with KCl, 10x Taq Buffer with (NH4)2SO4, and 25 mM MgCl2 solution) 100 units (5U/uL)
EP0402 Taq DNA polymerase (recombinant) (supplied with 10x Taq Buffer with KCl, 10x Taq Buffer with (NH4)2SO4, and 25 mM MgCl2 solution) 500 units (5U/uL)
EP0405 Taq DNA polymerase (recombinant) (supplied with 10x Taq Buffer with KCl, 10x Taq Buffer with (NH4)2SO4, and 25 mM MgCl2 solution) 5x 500 units (5U/uL)
EP0406 Taq DNA polymerase (recombinant) (supplied with 10x Taq Buffer with KCl, 10x Taq Buffer with (NH4)2SO4, and 25 mM MgCl2 solution) 10x 500 units (5U/uL)
EP0281 Taq DNA polymerase (native) (supplied with 10x Taq Buffer with KCl, 10x Taq Buffer with (NH4)2SO4, and 25 mM MgCl2 solution) 200 units (5U/uL)
EP0282 Taq DNA polymerase (native) (supplied with 10x Taq Buffer with KCl, 10x Taq Buffer with (NH4)2SO4, and 25 mM MgCl2 solution) 500 units (5U/uL)
10342-053 Taq DNA Polymerase, Recombinant 100 units
10342-020 Taq DNA Polymerase, Recombinant 500 units
18038-018 Taq DNA Polymerase, Native 100 units
18038-042 Taq DNA Polymerase, Native 500 units
18038-067 Taq DNA Polymerase, Native 3 × 500 units
18038-240 Taq DNA Polymerase, Native 5000 units
EP0071 Taq DNA Polymerase (native, with BSA) 200 units (5U/uL)
EP0072 Taq DNA Polymerase (native, with BSA) 500 units (5U/uL)
K0171 PCR Master Mix (2x) (contains Taq DNA polymerase (0.05 U/μl), reaction buffer, 4 mM MgCl2, 0.4 mM of each dNTP (dATP, dCTP, dGTP
and dTTP). Supplied with nuclease-free water		 200 reactions
K0172 PCR Master Mix (2x) (contains Taq DNA polymerase (0.05 U/μl), reaction buffer, 4 mM MgCl2, 0.4 mM of each dNTP (dATP, dCTP, dGTP
and dTTP). Supplied with nuclease-free water		 1000 reactions
F-501S DyNAzyme II DNA Polymerase 250 units (2U/uL)
F-501L DyNAzyme II DNA Polymerase 1000 units (2U/uL)
F-503L DyNAzyme II DNA Polymerase with Mg2+-free DyNAzyme Buffer and MgCl2 Solution 1000 units (2U/uL)
Enhanced PCR (higher sensitivity, longer PCR products and higher yields )
EP0701 Dream Taq DNA polymerase (2x DreamTaq buffer, dNTPs and 4 mM MgCl2. Supplied with nuclease-free water.) 100 units (5U/uL)
EP0702 Dream Taq DNA polymerase (2x DreamTaq buffer, dNTPs and 4 mM MgCl2. Supplied with nuclease-free water.) 500 units (5U/uL)
EP0703 Dream Taq DNA polymerase (2x DreamTaq buffer, dNTPs and 4 mM MgCl2. Supplied with nuclease-free water.) 5x 500 units (5U/uL)
EP0704 Dream Taq DNA polymerase (2x DreamTaq buffer, dNTPs and 4 mM MgCl2. Supplied with nuclease-free water.) 20x 500 units (5U/uL)
K1071 DreamTaq PCR Master Mix (2x) 200 reactions
K1072 DreamTaq PCR Master Mix (2x) 1000 reactions
EP0711 DreamTaq Green DNA Polymerase 100 units (5U/uL)
EP0712 DreamTaq Green DNA Polymerase 500 units (5U/uL)
EP0713 DreamTaq Green DNA Polymerase 5x 500 units (5U/uL)
EP0714 DreamTaq Green DNA Polymerase 20x 500 units (5U/uL)
K1081 DreamTaq Green PCR Master Mix (2x) 200 reactions
K1082 DreamTaq Green PCR Master Mix (2x) 1000 reactions
Hot start PCR
F-122S Phire Hot Start II DNA Polymerase 200 reactions
F-122L Phire Hot Start II DNA Polymerase 1000 reactions
EP0601 Maxima Hot Start Taq DNA Polymerase 100 units (5U/uL)
EP0602 Maxima Hot Start Taq DNA Polymerase 500 units (5U/uL)
EP0603 Maxima Hot Start Taq DNA Polymerase 5x 500 units (5U/uL)
K1051 Maxima Hot Start PCR Master Mix 100 reactions
K1061 Maxima Hot Start Green PCR Master Mix 100 reactions
K1062 Maxima Hot Start Green PCR Master Mix 500 reactions
10966-018 Platinum® Taq DNA Polymerase 100 reactions
10966026 Platinum® Taq DNA Polymerase 250 reactions
10966034 Platinum® Taq DNA Polymerase 500 reactions
10966083 Platinum® Taq DNA Polymerase 5000 reactions
4398813 AmpliTaq Gold 360 DNA Polymerase 100 units
4398823 AmpliTaq Gold 360 DNA Polymerase 250 units
4398833 AmpliTaq Gold 360 DNA Polymerase 1000 units
4398892 AmpliTaq Gold 360 DNA Polymerase 1500 units
4398894 AmpliTaq Gold 360 DNA Polymerase 3000 units
4398896 AmpliTaq Gold 360 DNA Polymerase 5000 units
4398898 AmpliTaq Gold 360 DNA Polymerase 25 x 1000 units
Hi-Fidelity PCR
F-549S Phusion Hot Start II High-Fidelity DNA polymerase 100 units (2U/uL)
F-549L Phusion Hot Start II High-Fidelity DNA polymerase 500 units (2U/uL)
11304011 Platinum Taq DNA Polymerase High Fidelity 100 reactions
11304-029 Platinum Taq DNA Polymerase High Fidelity 500 reactions
11304-102 Platinum Taq DNA Polymerase High Fidelity 5000 reactions
12344-024 AccuPrime Pfx DNA Polymerase 200 reactions
12344-032 AccuPrime Pfx DNA Polymerase 1000 reactions
12346-086 AccuPrime Taq DNA Polymerase High Fidelity 200 reactions
12346-094 AccuPrime Taq DNA Polymerase High Fidelity 1000 reactions
12355-012 Pfx50™ DNA Polymerase 100 reactions
12355-036 Pfx50™ DNA Polymerase 500 reactions
11708-013 Platinum® Pfx DNA Polymerase 100 reactions
11708-021 Platinum® Pfx DNA Polymerase 250 reactions
11708-039 Platinum® Pfx DNA Polymerase 500 reactions
F-553S Phusion High-Fidelity PCR Kit 50 reactions
F-553L Phusion High-Fidelity PCR Kit 200 reactions
F-531S Phusion High-Fidelity PCR Master Mix with HF Buffer 100 reactions
F-531L Phusion High-Fidelity PCR Master Mix with HF Buffer 500 reactions
F-532S Phusion High-Fidelity PCR Master Mix with HF Buffer 100 reactions
F-532L Phusion High-Fidelity PCR Master Mix with HF Buffer 500 reactions
Fast PCR
F-548S Phusion Flash High Fidelity Master Mix 100 reactions
F-548L Phusion Flash High Fidelity Master Mix 500 reactions
Long range PCR
11708-039 Platinum® Pfx DNA Polymerase 500 reactions
F-553S Phusion High-Fidelity PCR Kit 50 reactions
F-553L Phusion High-Fidelity PCR Kit 200 reactions
F-531S Phusion High-Fidelity PCR Master Mix with HF Buffer 100 reactions
F-531L Phusion High-Fidelity PCR Master Mix with HF Buffer 500 reactions
F-532S Phusion High-Fidelity PCR Master Mix with HF Buffer 100 reactions
Last Updated on Wednesday, 25 January 2012 09:42