Home Technical Support FAQ The vector solution in my TOPO cloning kit is yellow instead of pink, will it affect the TOPO reaction?
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Knowledge Base Article
The vector solution in my TOPO cloning kit is yellow instead of pink, will it affect the TOPO reaction?

The vector solution contains a pH indicator (phenol red) and should be pink at room temperature, if it is yellow it signifies an acidic pH but it will not affect the efficiency of the cloning. Even at a pH 2.0, TOPO vectors still maintain a high cloning efficiency. On the other hand if the vector turns blue when PCR product is added then the PCR product buffer is too basic (>9) and the number of transformed colonies will drop.

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Created : 2011-04-24 22:20:27, Last Modified : 2011-04-24 22:20:27
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