Biological Buffers

Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)

Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)

Used to prepare protein samples for denaturing gel electrophoresis with NuPAGE™ Novex™ gels. Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X) contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled

Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)

Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled.

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

Nuclease-free Water (not DEPC-treated) has been deionized, filtered into the final bottle, and autoclaved. It is ready to use and requires no preparation, mixing, or autoclaving.

Gibco™ HEPES (1M)

Gibco™ HEPES (1M)

A zwitterionic organic chemical buffering agent commonly used in cell culture media

Invitrogen™ NuPAGE™ Antioxidant

Invitrogen™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)

Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis

Invitrogen™ NuPAGE™ Transfer Buffer (20X)

Invitrogen™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting

Thermo Scientific™ DNA Gel Loading Dye (6X)

Thermo Scientific™ DNA Gel Loading Dye (6X)

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-free

Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-free

Invitrogen Molecular biology grade, 0.5 M EDTA, pH 8.0 solution is supplied in one bottle. The solution is certified RNase-free, economical, and ready-to-use.

Cytiva Pharmalyte™ Carrier Ampholytes

Cytiva Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. Cytiva Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.

Invitrogen™ 20X Bolt™ MES SDS Running Buffer

Invitrogen™ 20X Bolt™ MES SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats

Invitrogen™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X)

Invitrogen™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X)

Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels

Invitrogen™ MgCl<sub>2</sub> (1 M)

Invitrogen™ MgCl2 (1 M)

Molecular biology grade, Invitrogen 1M MgCl2 solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use.

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl.

Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer

Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Invitrogen™ GlycoBlue™ Coprecipitant (15 mg/mL)

Invitrogen™ GlycoBlue™ Coprecipitant (15 mg/mL)

GlycoBlue Coprecipitant consists of a blue dye covalently linked to glycogen, a branched chain carbohydrate, which is useful as a nucleic acid coprecipitant.

Invitrogen™ Recombinant Proteinase K Solution (20 mg/mL)

Invitrogen™ Recombinant Proteinase K Solution (20 mg/mL)

Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins.

Invitrogen™ DEPC-Treated Water

Invitrogen™ DEPC-Treated Water

Ambion DEPC-treated water is certified nuclease-free. DEPC-treated water is autoclaved pre- and post-packaging to ensure sterility and inactivation of DEPC.

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Invitrogen™ PureLink™ RNase A (20 mg/mL)

Invitrogen™ PureLink™ RNase A (20 mg/mL)

PureLink™ RNase A (ribonuclease A) is a bovine pancreatic ribonuclease that cleaves single-stranded RNA.

Invitrogen™ KCl (2 M), RNase-free

Invitrogen™ KCl (2 M), RNase-free

Invitrogen molecular biology grade, 2M KCl solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use.

Invitrogen™ RT-PCR Grade Water

Invitrogen™ RT-PCR Grade Water

RT-PCR Grade Water is certified free of nucleases, and free of nucleic acid contamination that may cause false-positive signals in RT-PCR. The RT-PCR Grade Water is ready to use and requires no preparation, mixing, or autoclaving.

Thermo Scientific™ SuperBlock™ Blocking Buffer

Thermo Scientific™ SuperBlock™ Blocking Buffer

SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.

Invitrogen™ UltraPure™ BSA (50 mg/mL)

Invitrogen™ UltraPure™ BSA (50 mg/mL)

Ambion Ultrapure BSA is a 'non-acetylated' BSA pure enough to use when DNA or RNA integrity is critical.

Thermo Scientific™ Blocker™ Casein

Thermo Scientific™ Blocker™ Casein

Blocker Casein is a ready-to-use solution of purified casein for blocking steps in western blot, ELISA, IHC, and nucleic acid detection methods.

Invitrogen™ Proteinase K Solution (20 mg/mL), RNA grade

Invitrogen™ Proteinase K Solution (20 mg/mL), RNA grade

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.

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