Cell Culture Media
Gibco™ N-2 Supplement (100X)
N-2 Supplement (100X)
Gibco™ DMEM, high glucose, no glutamine, no calcium
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.
Gibco™ DMEM/F-12, no phenol red
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Gibco™ Collagenase, Type I, powder
Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Gly-Pro, which is found with high frequency in collagen
Lonza™ Mammary Epithelial Growth Medium Bulletkit
Optimized for the growth of Mammary Epithelial cells in a serum-free environment. Mammary Epithelial Growth Medium Bulletkit is a readily available media kit giving researchers the confidence and the convenience of a system that works to support their cells.
Gibco™ Ham's F-12K (Kaighn's) Medium
Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment.
Gibco™ Essential 8™ Medium
Essential 8™ Medium
Gibco™ DMEM/F-12, GlutaMAX™ supplement
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Gibco™ Versene Solution
An EDTA solution for use as a gentle non-enzymatic cell dissociation reagent
Gibco™ MEM
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.
Gibco™ Human Endothelial SFM
Human Endothelial-SFM
Gibco™ DMEM/F-12, HEPES
Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Cytiva HyClone™ RPMI 1640 Media
Designed to support cell culture growth and biomanufacturing processes.
Gibco™ Opti-MEM™ I Reduced Serum Medium
Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology.
Gibco™ DMEM, no glucose
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
Gibco™ Medium 199, Earle's Salts
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.
Gibco™ B-27™ Supplement (50X), custom
B-27™ Supplement (50X), custom
Gibco™ MEM, no glutamine
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells.
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MP Biomedicals™ Medium 199 (modified) with Hanks' Salts with Glutamine without Sodium Bicarbonate
Medium 199 (modified) with Hanks' Salts with Glutamine without Sodium Bicarbonate
Gibco™ DMEM/F-12, HEPES, no phenol red
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Gibco™ Advanced MEM
Advanced MEM (Minimum Essential medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.
Corning™ Insectagro™ Sf9 Serum-free/Protein-free Medium, 1X, With L-glutamine
Formulated to support propagation of Sf9 insect cells in culture.
Gibco™ Recovery™ Cell Culture Freezing Medium
Complete, ready-to-use freezing medium for cryopreservation of a wide variety of mammalian cells, including CHO-S, CHO-K1, HEK 293, Jurkat, and NIH 3T3
Gibco™ Glucose Solution
Gibco™ Glucose Solution contains D-glucose at a concentration of 200 g/L and is used as a serum-free medium supplement suitable for cell culture.
Gibco™ MEM α, no nucleosides
MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.
Gibco™ AmnioMAX™-II Complete Medium
Fully-supplemented medium developed for short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures
Gibco™ DMEM, high glucose, no glutamine
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
Gibco™ Expi293™ Expression Medium
Expi293™ Expression Medium
Cell culture media support the growth and maintenance of a variety of mammalian and other cell lines in vitro, as either anchorage-dependent or suspension cultures. Cell culture offers convenience, control, consistency and reproducibility of results, especially when using clonal cells.
Cell culture has multiple applications in cellular and molecular biology:
- Studying normal cell physiology and biochemistry
- Industrial bioprocess/bioreactor manufacturing of vaccines, therapeutic proteins and monoclonal antibodies using recombinant, high-yield protein expression systems
- Pharmaceutical screening and development, including drug metabolism and toxicology
- Other emerging applications in stem cell culture, immunotherapy and cell therapy
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
- Classical mammalian cell culture media (DMEM, MEM, RPMI 1640, McCoy’s, Ham’s, BME and EBSS) contain all the nutrients that cells need, such as amino acids, minerals, vitamins and glucose
- Individual cell culture reagents and supplements, including growth factors (e.g. pyruvate), antibiotics, hormones, cryopreservation media, cell dissociation reagents and animal-derived sera (e.g., FBS foetal bovine serum) which contain a ready-made natural mixture of nutrients
- Speciality media, including stem cell culture media, transfection media, and serum-free or animal-derived component-free (ACDF) additives
- Ancillary buffers and process liquids, including balanced salt solutions (PBS phosphate buffered saline, Hank’s HBSS, Earle’s EBBS), sodium bicarbonate and HEPES buffers, phenol red, cell culture grade water, and adherent cell microcarriers
Cell culture media are typically supplied as presterilised ready-to-use liquids, concentrates, powders or other solids in bottles, or else as single-use sealed bags for integration into compatible cell culture harvesting systems.
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories: