Enzymes and Inhibitors

Thermo Scientific™ I-SceI (10 U/μL)

Thermo Scientific™ I-SceI (10 U/μL)

Cut at TAGGGATAA^CAGGGTAAT sites with I-SceI homing enzyme, which performs best at 37°C in Tango buffer.

Thermo Scientific™ Reaction Buffer with MgCl<sub>2</sub> for DNase I (10X)

Thermo Scientific™ Reaction Buffer with MgCl2 for DNase I (10X)

Optimize reaction condions for RNase-free DNase I with this 10X reaction Tris-HCl (pH 7.5) buffer containing MgCl2.

Thermo Scientific™ FastDigest LguI (IIs class)

Thermo Scientific™ FastDigest LguI (IIs class)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Thermo Scientific™ FastDigest NotI

Thermo Scientific™ FastDigest NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI).

Thermo Scientific™ FastDigest™ NheI: PROMO

Thermo Scientific™ FastDigest™ NheI: PROMO

Thermo Scientific FastDigest NheI restriction enzyme recognizes G^CTAGC site and cuts best at 37°C in 5 to 15 minutes using universal FastDigest Buffer. Isoschizomers: AsuNHI, BmtI.

Thermo Scientific™ T4 DNA Ligase Buffer (10X)

Thermo Scientific™ T4 DNA Ligase Buffer (10X)

Safely ligate samples with this buffer, for use with T4 DNA Ligase and tested for the absence of endo- and exodeoxyribonucleases and ribonucleases.

Thermo Scientific™ FastDigest BpiI (IIs class)

Thermo Scientific™ FastDigest BpiI (IIs class)

Cut at GAAGAC(2/6)^ sites with Thermo Scientific™ FastDigest™ BbsI (BpiI), which performs best using universal FastDigest Buffer (Isoschizomers: BpuAI, BstV2I).

Thermo Scientific™ Bst1107I (BstZ17I) (10 U/μL)

Thermo Scientific™ Bst1107I (BstZ17I) (10 U/μL)

Cut at GTA^TAC sites with Bst1107I (BstZ17I) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: BssNAI, BstZ17I).

Thermo Scientific™ FastDigest KpnI

Thermo Scientific™ FastDigest KpnI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Thermo Scientific™ BclI (10 U/μL)

Thermo Scientific™ BclI (10 U/μL)

Cut at T^GATCA sites with BclI restriction enzyme, which performs best at 55°C in G buffer (Isoschizomers: FbaI, Ksp22I).

Thermo Scientific™ ATP Solution (100 mM)

Thermo Scientific™ ATP Solution (100 mM)

Thermo Scientific ATP (adenosine 5'-triphosphate) is an extremely stable nucleotide, and it is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.

Thermo Scientific™ TAE Buffer (Tris-acetate-EDTA) (50X)

Thermo Scientific™ TAE Buffer (Tris-acetate-EDTA) (50X)

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).

Thermo Scientific™ FastDigest EcoRI

Thermo Scientific™ FastDigest EcoRI

The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Thermo Scientific™ AarI (2 U/μL)

Thermo Scientific™ AarI (2 U/μL)

Cut at CACCTGC(4/8)^ sites with AarI restriction enzyme, which performs best at 37°C in its own unique (+oligo) buffer.

Thermo Scientific™ FastDigest BcuI

Thermo Scientific™ FastDigest BcuI

FastDigest SpeI (BcuI) restriction enzyme recognizes A^CTAGT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AhlI).

Thermo Scientific™ Bovine Serum Albumin (BSA)

Thermo Scientific™ Bovine Serum Albumin (BSA)

Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin.

Thermo Scientific™ PvuII (10 U/μL)

Thermo Scientific™ PvuII (10 U/μL)

The PvuII restriction enzyme recognizes CAG^CTG sites and cuts best at 37°C in G buffer.

Thermo Scientific™ SdaI (SbfI) (10 U/μL)

Thermo Scientific™ SdaI (SbfI) (10 U/μL)

The SdaI (SbfI) restriction enzyme recognizes CCTGCA^GG sites and cuts best at 37°C in its own unique buffer (Isoschizomers: SbfI, Sse8387I).

Thermo Scientific™ FastDigest Esp3I (IIs class)

Thermo Scientific™ FastDigest Esp3I (IIs class)

Cut at CGTCTC(1/5)^ sites with Thermo Scientific™ FastDigest™ BsmBI (Esp3I), which peforms best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Thermo Scientific™ BspTI (AflII) (10 U/μL)

Thermo Scientific™ BspTI (AflII) (10 U/μL)

Cut at C^TTAAG sites with BspTI (AflII) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AflII, BfrI, Bst98I, BstAFI, MspCI, Vha464I).

Invitrogen™ Anza™ 28 MluI

Invitrogen™ Anza™ 28 MluI

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.

Thermo Scientific™ GTP Solution (100 mM)

Thermo Scientific™ GTP Solution (100 mM)

Thermo Scientific GTP (guanosine 5'-triphosphate) is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.

Thermo Scientific™ Eam1105I (AhdI) (10 U/μL)

Thermo Scientific™ Eam1105I (AhdI) (10 U/μL)

Cut at GACNNN^NNGTC sites with Eam1105I (AhdI) restriction enzyme, which performs best at 37°C in its own unique buffer (Isoschizomers: AhdI, AspEI, BmeRI, DriI, EclHKI).

Invitrogen™ Anza™ 35 Eco47III

Invitrogen™ Anza™ 35 Eco47III

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.

Thermo Scientific™ FastDigest PvuII

Thermo Scientific™ FastDigest PvuII

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Thermo Scientific™ FastDigest PdmI

Thermo Scientific™ FastDigest PdmI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Invitrogen™ Anza™ 121 Bsp120I

Invitrogen™ Anza™ 121 Bsp120I

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.

Thermo Scientific™ FastDigest FspBI

Thermo Scientific™ FastDigest FspBI

Cut at C^TAG sites with Thermo Scientific™ FastDigest™ BfaI (FspBI), which performs best using universal FastDigest Buffer (Isoschizomers: BfaI, MaeI, XspI).

Thermo Scientific™ Alw44I (10 U/μL)

Thermo Scientific™ Alw44I (10 U/μL)

Cut at G^TGCAC sites with Alw44I (ApaLI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: ApaLI, VneI).

Thermo Scientific™ FastDigest Hin6I

Thermo Scientific™ FastDigest Hin6I

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

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