Electrophoresis, Western Blotting and ELISA

Invitrogen™ SeeBlue™ Plus2 Pre-stained Protein Standard

Invitrogen™ SeeBlue™ Plus2 Pre-stained Protein Standard

Allows you to easily visualize protein molecular weight ranges during electrophoresis and quickly evaluate Western transfer efficiency

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled

Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)

Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)

Used to prepare protein samples for denaturing gel electrophoresis with NuPAGE™ Novex™ gels. Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X) contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.

Invitrogen™ SimplyBlue™ SafeStain

Invitrogen™ SimplyBlue™ SafeStain

SimplyBlue SafeStain is a ready-to-use, fast, sensitive, and safe Coomassie G-250 stain for visualizing protein bands on polyacrylamide gels and on dry PVDF membranes.

Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)

Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis

Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)

Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled.

Thermo Scientific™ 1-Step™ ABTS Substrate Solution

Thermo Scientific™ 1-Step™ ABTS Substrate Solution

Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.

Merck Immobilon™-FL PVDF Membranes

Merck Immobilon™-FL PVDF Membranes

Designed specifically for Western blots and protein sequencing. Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45 μm pores and have lower background fluorescence than other membranes. Available in 10 and 20 cm squares and a 3.75 m roll.

Cytiva Amersham™ Hyperfilm™ ECL

Cytiva Amersham™ Hyperfilm™ ECL

Use with all blue and green light chemiluminescent systems. Cytiva Amersham™ Hyperfilm™ ECL is constructed from a clear base coated on both sides with a photographic emulsion that is protected by an anti-scratch layer.

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

Invitrogen™ HiMark™ Pre-stained Protein Standard

Invitrogen™ HiMark™ Pre-stained Protein Standard

Designed for analysis of high molecular weight proteins on NuPAGE™ Novex™ Tris-Acetate gels

Merck Immobilon™ Western Chemiluminescent HRP Substrate (ECL)

Merck Immobilon™ Western Chemiluminescent HRP Substrate (ECL)

Provides high sensitivity over a broad detection range at an economical price

Invitrogen™ NuPAGE™ 10%, Bis-Tris, 1.0–1.5 mm, Mini Protein Gels

Invitrogen™ NuPAGE™ 10%, Bis-Tris, 1.0–1.5 mm, Mini Protein Gels

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial.

Thermo Scientific™ HRP-Conjugated Streptavidin

Thermo Scientific™ HRP-Conjugated Streptavidin

Streptavidin from Streptomyces avidinii conjugated to highly purified horseradish peroxidase (HRP)

Invitrogen™ NuPAGE™ Antioxidant

Invitrogen™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

Invitrogen™ DryEase™ Mini Cellophane

Invitrogen™ DryEase™ Mini Cellophane

Component of DryEase Mini-Gel Drying System

Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa

Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa

Mixture of 9 blue-, orange- and green-stained proteins (10 to 250kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Thermo Scientific™ Pierce™ Streptavidin

Thermo Scientific™ Pierce™ Streptavidin

Purified recombinant streptavidin enables detection of biotinylated antibodies and other probes in a variety of standard assay methods including western blotting, ELISA, immunohistochemistry, and fluorescence imaging.

Invitrogen™ SureCast™ Gel Handcast Bundle A - Hardware and Reagents

Invitrogen™ SureCast™ Gel Handcast Bundle A - Hardware and Reagents

Pour your own polyacrylamide gels easily and confidently with a SureCast™ Gel Handcast Bundle A. The SureCast Handcast System can be used to create polyacrylamide gels using SureCast Handcast Reagents as well as any other popular polyacrylamide gel casting reagents.

Invitrogen™ Mini Gel Tank

Invitrogen™ Mini Gel Tank

Compatible with a variety of Novex™ gels, NuPAGE™ gels, and Bolt™ Bis-Tris Plus gels

Invitrogen™ Bolt™ Bis-Tris Plus Mini Protein Gels, 4‐12%, 1.0 mm, WedgeWell™ format

Invitrogen™ Bolt™ Bis-Tris Plus Mini Protein Gels, 4‐12%, 1.0 mm, WedgeWell™ format

Invitrogen Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of a wide range of protein sizes under denaturing conditions.

Thermo Scientific™ Pierce™ CN/DAB Substrate Kit

Thermo Scientific™ Pierce™ CN/DAB Substrate Kit

Combines chloronaphthol- and diaminobenzidine-based HRP detection for Western blot and tissue staining methods.

Invitrogen™ iBlot™ 2 Transfer Stacks, nitrocellulose, regular size

Invitrogen™ iBlot™ 2 Transfer Stacks, nitrocellulose, regular size

Used to transfer proteins with the iBlot 2 Gel Transfer Device

Thermo Scientific™ 1-Step™ TMB-Blotting Substrate Solution

Thermo Scientific™ 1-Step™ TMB-Blotting Substrate Solution

Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.

Thermo Scientific™ PageRuler™ Unstained Protein Ladder

Thermo Scientific™ PageRuler™ Unstained Protein Ladder

A mixture of seven native proteins (14.4 to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

Thermo Scientific™ Pierce™ Unstained Protein MW Marker

Thermo Scientific™ Pierce™ Unstained Protein MW Marker

A mixture of seven native proteins (14.4kDa to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

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